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. 2022 Feb 17;15(2):241. doi: 10.3390/ph15020241

Figure 2.

Figure 2

TGEV infection did not activate IR/IGF-1R signaling but induced JAK1 activation in the ST cell. ST cells were infected by TGEV at an MOI of 7 or respectively stimulated with IGF-1 (10 nM) (A) or insulin (100 nM) (B). The resulting cell lysates, harvested at the indicated time points of 0.5 h, 1 h, 3 h, and 6 h, were subjected to western analyses with antibodies against p-JAK1, JAK1, p-IGF-1R, IGF-1R, p-insulin R, insulin R or the internal loading controls vinculin or β-actin, respectively. *, p < 0.05; **, p < 0.01. Results shown are averages ± SD from three independent experiments.