Figure 3.

AG1024 diminished TGEV induced JAK1 activation/phosphorylation and inhibited TGEV activity in a dose-dependent manner. ST cells were pretreated with various concentrations of AG1024 or PPP respectively for 1 h prior to infection by TGEV at an MOI of 7. The resulting cells were harvested at 6 h.p.i. and then subjected to western analyses with antibodies against p-JAK1, JAK1, N, or an internal loading control GAPDH, respectively. *, p < 0.05; **, p < 0.01. Results shown are averages ± SD from three independent experiments.