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. 2022 Feb 17;15(2):241. doi: 10.3390/ph15020241

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Additive effect of AG1024 and ouabain in anti-TGEV activity and JAK1-diminishment. (A) Combined treatment of AG1024 and ouabain profoundly and additively reduced TGEV infection as assayed by IFA in ST cells. (B) Combined treatments of AG1024 and ouabain additively diminish JAK1 protein levels. TGEV (MOI:7) infected ST cells were treated with compounds as indicated, and the resulting cells were subjected to IFA or harvested at 6 h.p.i. for subsequent western analyses. The results obtained from IFA (A(a,b)) or western B(a) were further analyzed by SynergyFinder(A(c),B(b,c)). **, p < 0.01. Results shown are averages ± SD from three independent experiments.