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. 2022 Feb 11;13:820843. doi: 10.3389/fimmu.2022.820843

Figure 1.

Figure 1

TRAP and Hoechst staining on day 28. Monocultures of PBMCs cultivated with (A) and without (B) additional M-CSF/RANKL and (C) Co-cultures of PBMCs/PPFs. Different sizes of multiple TRAP positive multinucleated cells in (C) additionally surrounded by small spindle-shaped cells. Photos were taken via light microscope (left), a fluorescence filter (middle), and combined (right). Scale bar = 100 μm.