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Resorption lacunae on dentin after staining with toluidine blue. Resorption lacunae on dentin after staining with toluidine blue. Lack of resorption pits in monolayer PBMC/PPFs co-cultures (A), even when cultivated in the presence of additional MCSF and RANKL (B). PBMC monocultures stimulated with MCSF and RANKL, cultivated in monolayer (C) or on transwell membranes [and transferred on dentin (D)], showed traces of osteolysis.
