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. 2022 Feb 11;13:820843. doi: 10.3389/fimmu.2022.820843

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Copyright © 2022 Schluessel, Hartmann, Koehler, Beck, Redeker, Saller, Akova, Krebs, Holzapfel and Mayer-Wagner

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mRNA expression of PPF monocultures and PBMC/PPF co-cultures (day 13 and 20). mRNA expression of PPF monocultures and PBMC/PPF co-cultures. Relative mRNA Relative mRNA expression (A) RANKL, (B) OPG, (C) RANK, (D) MCSF, (E) OCN, (F) CTSK, (G) TRAP and (H) TNFa (normalized to housekeeping gene EF1a) in co-cultures of PPF and PBMCs in monolayer (ML, n = 16) and transwell (TW, n = 16) as well as in monocultures of PPF in monolayer (n = 8) on day 13 and day 20. Bands inside the boxes indicate group medians,crosses indicate group means. End of whiskers represent minimum and maximum values. Baseline refers to relative mRNA expression of original periosteolytic tissue (n=8). p values are indicated with * (p ≤ 0.05), ** (p ≤ 0.01),*** (p ≤ 0.001), ns, not significant.