Table 3.
Extract | Fraction | Peak 1 a | Peak 2 | Peak 3 | Peak 4 | Total Yield b |
---|---|---|---|---|---|---|
CT50 | F14 | 1.64 | 9.72 | 15.91 | 3.52 | 30.79 |
F15 | 2.85 | 14.62 | 21.07 | 4.07 | 42.62 | |
F16 | 2.89 | 12.52 | 16.32 | 3.51 | 35.24 | |
F17 | 0.79 | 3.81 | 5.87 | 1.10 | 11.56 | |
F18 | 1.87 | 8.93 | 14.32 | 2.66 | 27.78 | |
F19 | 1.34 | 5.92 | 9.24 | 1.86 | 18.35 | |
F20 | 0.20 | 1.10 | 1.75 | 0.34 | 3.40 | |
F21 | 0.40 | 1.68 | 2.35 | 0.52 | 4.95 | |
F22 | 1.64 | 9.72 | 15.91 | 3.52 | 30.79 | |
CM50 | F16 | 1.21 | 11.34 | 20.73 | 1.99 | 35.27 |
F17 | 2.42 | 20.48 | 21.16 | 2.83 | 46.89 | |
F18 | 1.60 | 11.47 | 15.43 | 1.88 | 30.37 | |
F19 | 1.31 | 9.06 | 10.69 | 1.44 | 22.50 | |
F20 | 0.67 | 5.28 | 5.29 | 0.72 | 11.96 | |
F21 | 0.49 | 3.34 | 4.23 | 0.58 | 8.64 | |
F22 | 1.25 | 9.93 | 10.04 | 1.45 | 22.67 | |
F23 | 0.68 | 5.50 | 4.79 | 0.73 | 11.70 | |
F24 | 0.36 | 2.32 | 2.22 | 0.35 | 5.25 |
a 1: Igalan ([114]; not unequivocally confirmed by 1H NMR (proton nuclear magnetic resonance) due to substantial amounts of lipids in the subfraction); 2: Isoalantolactone (confirmed with standard); 3: Alantolactone (confirmed with standard); 4: unseparated mixture of dugesialactone and alloalantolactone ([114] alloalantolactone presence confirmed by 1H NMR). b Except for the eudesmanolides, fatty acids and, as it was shown by 1H NMR analysis, complex mixture of lipids without UV/Vis absorption may constitute the sample.