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. 2022 Feb 11;23(4):2012. doi: 10.3390/ijms23042012

Figure 4.

Figure 4

LA attenuated the apoptotic DRG neurons. (A) The hindpaw-withdrawal threshold (g) was detected using calibrated forceps test at day 3, 7, and 14 after CCI. LA serves as a TSLP inhibitor. CCI decreased the paw-withdrawal threshold in the ipsilateral (ipsi) injured side as compared to the contralateral (contra) side in rats. Values are presented as means ± s.e.m. (n = 6 rats). * p < 0.05, compared to CCI group (contra), # p < 0.05, compared to CCI group (ipsi); ANOVA test. LA increased the paw-withdrawal threshold in the ipsilateral side. (B) Apoptotic neurons were measured when DRG neurons were double-labeled (yellow) with TUNEL (green) and NeuN (red). Pairs of merged images are shown in the right panels. White arrows indicate apoptotic neurons. Scale bars represent 100 μm. (C) Merge immunoreactivities were parallel-measured in ipsilateral and contralateral sides of DRG neurons at 7 and 14 days. This expression of apoptotic neurons in the ipsilateral (ipsi) injured side increased after nerve injury and decreased after LA administration as compared to that in the contralateral (contra) side. Values are presented as means ± s.e.m. (n = 6 rats). * p < 0.05, compared to LA administration group, Students’ t test.