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. 2022 Feb 21;11(4):569. doi: 10.3390/plants11040569

Figure 5.

Figure 5

Methionine content analysis of identified peptides by mass spectrometry after 1D SDS-PAGE (A), 2Dd-CNBr carried out in control (non-oxidized) (B) and oxidized (HOCl) (C) conditions. (A) 5968 peptides were identified by two independent mass spectrometry analyses; 1348 peptides contained at least one methionine including 757 and 1109 oxidized and reduced Met residues, respectively (see Table S6). (B) 704 peptides were identified and 29 contained at least an oxidized (+15.99491 Da) and 23 a modified Met residue (−29.9928 Da or −48.0034 Da) (see Table S3). (C) 378 peptides were identified and 110 contained at least one oxidized Met residue (+15.99491 Da) (see Table S5). HS, homoserine.