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Dot immunoblots detect the presence of transient AβOa in developing retina. Soluble extracts of retinas were obtained from embryonic chicks at ages E7, E14, and E20 and increasing doses were applied to the filter. AβOs were detected using the mouse monoclonal antibody NU2. A major increase was seen between E7 and E14 followed by a prominent decrease between E14 and E20. N = 3 for each embryonic age.
