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. 2022 Feb 17;23(4):2208. doi: 10.3390/ijms23042208

Figure 4.

Figure 4

Retina AβOs comprise proteoforms differentially recognized on Western blots by monoclonal antibodies NU2, ACU193, and NU4. Soluble extracts from three embryonic retinas were obtained at E14 and separated using SDS-PAGE (Tris-glycine gel) followed by transfer to a nitrocellulose membrane. This was repeated twice for a total of nine different retinas (1–9) on three separate membranes. AβOs were identified using NU2 (A; 1–3), ACU193 (B; 4–6), or NU4 (C; 7–9). AβOs identified with NU2 were most prominent as a doublet at ~45 kDa with a minor band just above 37 kDa. AβOs identified with ACU193 had prominent bands at ~72 kDa and a doublet at ~100 kDa. AβOs identified with NU4 had prominent bands just above ~37 kDa and ~100 kDa, with fainter bands at ~14 kDa, 45 kDa, and 125 kDa. The ability of NU2, ACU193, and NU4 to distinguish these distinct SDS-stable AβO proteoforms was observed in three separate experiments.