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. 2022 Feb 17;23(4):2208. doi: 10.3390/ijms23042208

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cultured shell-free embryos resemble embryos maintained in ovo. Embryos were cultured shell-less, as described in the Materials and Methods section, and development was compared with embryos maintained in ovo. (A) Embryo incubated in ovo to E14 (from Hamburger Hamilton [83]), Copyright 1951 John Wiley and Sons. (D) Embryo incubated in shell-free culture to E14. (B) NU4-targeted AβOs (red) and (C) PHF-1-targeted pTau (green) in retina of E14 in ovo embryo. (E) NU4-targeted AβOs (red) and (F) PHF-1-targeted pTau (green) in retina of E14 ex ovo embryo.