Figure 12.

Laminar disruption by injected ACU193 as well as BACE inhibitor indicates a role for embryonic AβOs in retina histogenesis. (A) Further examples of dysplasia from ex ovo embryos injected with β-Secretase inhibitor (from embryos shown in Figure 11). Retinas were stained with DAPI (grayscale) to identify cell nuclei. Laminar disruptions of various appearance extend across all retinal layers. Scale bar is 25 µm. (B,C) Ex ovo embryo eyes were injected with ACU193 at embryonic day 9 and processed for imaging with DAPI at day 11. Dysplasia was evident in the peripheral retina and showed folds similar to those found after BACE inhibitor injections. The red box in (B) identifies one such dysplasia. Scale bar in (B) is 1000 µm. Scale bar in (C) is 25 µm. (D,E) No disruption was observed in either control eyes injected with non-specific human IgG (D) or non-injected eyes (E). Scale bars are 1000 μm. N = 2 for each condition.