Figure 2.

Alterations of phosphatidylcholine (PC aa) levels in different cell lines (SH-SY5Y and N2a) in the presence of the analyzed MTXs caffeine (C), theophylline (TP), pentoxifylline (P), theobromine (TB) and propentofylline (PPF). (A) PC aa total. Relative changes of PC aa species in comparison to cells treated with the solvent control are presented in volcano plots for each examined cell line. Lipid species with changes smaller than the standard deviation (SD) are marked in grey, while those changed greater than the SD are marked in black. p-values were calculated using Dunnett’s post-hoc test for each lipid class and statistical significance was set at p ≤ 0.05 (horizontal line in volcano plots). Below the volcano plots, the relative changes are presented in boxplots for the corresponding cell line as fold changes to the solvent control. (B) SH-SY5Y cells. Distribution of saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA), as well as short-, medium- and long-chain PC aa species in SH-SY5Y cells treated with PPF. Short-chain was defined as < C32:X, medium-chain as C32:X–C36:X and long-chain as > C36:X for PC aa throughout the manuscript. (C) N2a cells. Distribution of SFA, MUFA and PUFA as well as short-, medium- and long-chain PC aa species in N2a cells treated with C, TP, and TB. (B,C) are shown as boxplots in Supplemental Figure S2. (* p ≤ 0.05, *** p ≤ 0.001).