Figure 1.

Venn diagram of the three gene lists used for the selection of miR-183 candidate direct target genes. 1: Genes downregulated and upregulated upon transfection of miR-183 mimic or inhibitor, respectively, identified by gene expression microarrays of the DU145 and LNCaP cell lines (fold change ≥ 1.25 in at least two of the four conditions assessed). 2: Argonaut-associated transcripts containing the 6-mer (2–7 nt) sequence complementary to the miR-183 seed extracted from prostate cell lines in the AGO-PAR-CLIP experiments performed by Hamilton et al. [31] (at least one read in three out of five cell lines published). 3: Transcripts showing a negative Spearman correlation with miR-183 expression (p-value < 0.05, r² < 0) in the PRAD-TCGA samples.