Table 3.
Primary and secondary antibodies used in the immunohistochemical studies performed, showing the dilutions used and the protocol specifications.
| Antigen | Species | Dilution | Provider | Protocol Specifications |
|---|---|---|---|---|
| ILK | Rabbit | 1:50 | Abcam (ab52,480) | 10 mM Sodium citrate pH = 6 before incubation with blocking solution |
| E-Cad | Mouse | 1:250 | Vitro (MAD-000761QD-3/V) |
- |
| Cad-17 | Rabbit | 1:250 | Vitro (MAD-000737QD-3/V) |
- |
| Cad-6 | Mouse | 1:250 | Vitro (MAD-000582QD-3/V) |
- |
| IgG (Mouse) |
Goat | 1:300 | Sigma-Aldrich (F2012/045K6072) |
- |
| IgG (Rabbit) |
Mouse | 1:1000 | Sigma-Aldrich (RG-96/B5283) |
- |