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. 2022 Jan 23;14(2):86. doi: 10.3390/toxins14020086

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Inhibitory effect of Trichoderma spp. metabolites on the growth of A. flavus Af-9. The isolates of Trichoderma spp. were grown on sterilized cellophane sheets in Petri dishes containing Czapek Dox-Agar (A) or Czapek Dox-Agar supplemented with 2% peanut flour (B) (see text for more details) for 5 or 3 days, respectively. After removal of the cellophane sheet, the dishes were inoculated with Af-9 and incubated at 25 °C with 12/12 photoperiod for 6 days. Values are the means ± SD (n = 3) of the percent reduction of colony diameter with respect to control. Asterisks indicate statistically significant values at p < 0.05 (*), p < 0.01 (**) or = p < 0.001 (***) by one-way ANOVA.