Figure 3.
Supervised multivariate clustering model demonstrating ethnicity specific oxylipins profile discriminates Hispanic (HIS) and Caucasian (CAU) with non-alcoholic steatohepatitis (NASH) without advanced fibrosis. Score plot for NASH-HIS (red) vs. NASH-CAU (blue) when excluding stage 3 and 4 fibrosis. Light color represents subjects from primary cohort and dark color represents secondary cohort. The model was performed on all profiled lipids. Lipid mediators are colored according to metabolizing enzyme pathways, lipoxygenase, and autoxidation (red); cytochrome p450 epoxygenase (brown); and soluble epoxide hydrolase (green); cyclooxygenase (blue); other (black). The model was validated with leave-one-out cross validation. The Q2 and R2 are 0.99 and 0.98, respectively. Details on variable importance into the projection scores are shown in Table S4. NASH (n = 9 HIS and 13 CAU). DiHETE, dihydroxyeicosatetraenoic acid; DiHETrE, dihydroxyeicosatrienoic acid; DiHODE, dihydroxyoctadecadienoic acid; DiHOME, dihydroxyoctadecenoic acid; Ep-KODE, epoxyoxooctadecenoic acid; EpETE, epoxyeicosatetraenoic acid; EpODE, epoxyoctadecadienoic acid; EpOME, epoxyoctadecenoic acid; HETE, hydroxyeicosatetraenoic acid; HOTE, hydroxyoctadecatrienoic acid; KETE, keto-eicosatetraenoic; KODE, keto-octadecadienoic acid; PGE, prostaglandin E; TriHOME, trihydroxyoctadecaenoic acid; TXB, thromboxane.