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. 2022 Feb 9;12(2):165. doi: 10.3390/metabo12020165

Figure 1.

Figure 1

Overview of performance score and selectivity under chromatographic conditions. (a) Individual score of column performance for polar metabolites from different classes of compounds. (b) Column selectivity and chromatographic peak performance of representative metabolites from different classes under five different LC method conditions. Anomer mutarotation causes the glucose signal to split into two separate peaks as shown on both columns. The arrows represent the zoom figures. Blue: ZIC-c HILIC at pH 3; Red: ZIC-c HILIC at pH 7; Green: BEH Amide at pH 3; Purple: BEH Amide at pH 7; Yellow: BEH Amide at pH 10.