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Isolation and morphology of DFAT cells. (a) After digestion and centrifugation of small pieces of fat tissue, the isolated unicolor mature adipocytes adhered to the top inner surface of a culture flask and generated fibroblast-like cells. (b) Microscopic view of primary cultured DFAT cells harvested from MMPs during the ceiling culture divide asymmetrically and generate fibroblast-like cells.
