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Autoimmune reactivity of wild-type and variants of CR3022 (CR3022, S103F–S33R and S103Y–S33R) to human Hep2 cells with TNM002 as negative control and LC0042 as a positive control. Green fluorescence was photographed under an EVOS fluorescence microscope. The three antibodies to be tested (CR3022, S103F–S33R and S103Y–S33R) and negative control antibody TNM002, positive control antibody LC0042 were added at three concentrations of 25 μg/mL, 50 μg/mL and 100 μg/mL, respectively.
