Table 2.
Primers for mutagenesis.
| Plasmid Name | Template | Primers for Mutagenesis |
|---|---|---|
| ΔARM-Rex | Rex-pME-FLAG | ΔARM-Rex-Top: CCTCGAGTGGCCCACTTCCCAGGGT ΔARM-Rex-Bottom: GTGGGCCACTCGAGGAATTCCTTGTC |
| Δaa20-56-Rex | Rex-pME-FLAG | Δaa20-56-Rex-Top: AACACCACCCGCCTACATCGTCACG Δaa20-56-Rex-Botom: TAGGCGGGTGGTGTTGGTGGTCTTTT |
| ΔN-MD-Rex | Rex-pME-FLAG | ΔN-MD-Rex-Top: TGTTCGGCCTGTCCAGAGCATCAGA ΔN-MD-Rex-Bottom: TGGACAGGCCGAACATAGTCCCCCAG |
| ΔNES-Rex | Rex-pME-FLAG | ΔNES-Rex-Top: CCCCATCGAGAGAACCTCTAAGACCC ΔNES-Rex-Bottom: GTTCTCTCGATGGGGTCCCAGGTGA |
| ΔC-MD-Rex | Rex-pME-FLAG | ΔC-MD-Rex-Top: AAGACCCTCCAGGCCATGCGCAAAT ΔC-MD-Rex-Bottom: GGCCTGGAGGGTCTTAGAGGTTCTCT |
| ΔARM/NES-Rex | ΔNES-Rex | ΔARM-Rex-Top: CCTCGAGTGGCCCACTTCCCAGGGT ΔARM-Rex-Bottom: GTGGGCCACTCGAGGAATTCCTTGTC |
| ΔN/C-MD-Rex | ΔC-MD-Rex | ΔN-MD-Rex-Top: TGTTCGGCCTGTCCAGAGCATCAGA ΔN-MD-Rex-Bottom: TGGACAGGCCGAACATAGTCCCCCAG |
| Δaa125-139-Rex | Rex-pME-FLAG | Rex(Δaa125-139)-Top: TCCTTCCAACCCACCCTTGGGCAGC Rex(Δaa125-139)-Bottom: GGTGGGTTGGAAGGAGGGTGGAATGT |
| Δaa125-149-Rex | Rex-pME-FLAG | Rex(Δaa125-149)-Top: TCCTTCCTGTCTTTTCCAGACCCCG Rex(Δaa125-149)-Bottom: AAAAGACAGGAAGGAGGGTGGAATGT |
| Δaa125-159-Rex | Rex-pME-FLAG | Rex(Δaa125-159)-Top: TCCTTCCAAAACCTGTACACCCTCT Rex(Δaa125-159)-Bottom: CAGGTTTTGGAAGGAGGGTGGAATGT |
| Δaa125-169-Rex | Rex-pME-FLAG | Rex(Δaa125-169) -Top: CTCCTTCCTTGTCTGCATGTACCTCT Rex(Δaa125-169)-Bottom: CAGACAAGGAAGGAGGGTGGAATGT |
| Δaa170-189-Rex | Rex-pME-FLAG | Rex(Δaa170-189)-Top: AGGCTCCGTGAACTAGTCTAGAGAAA Rex(Δaa170-189)-Bottom: TAGTTCACGGAGCCTCCCCAGAGGG |
| Δaa160-189-Rex | Rex-pME-FLAG | Rex(Δaa160-189)-Top: CCGGCCCCTGAACTAGTCTAGAGAAA Rex(Δaa160-189)-Bottom: TAGTTCAGGGGCCGGAGTCCGGGGT |
| Δaa150-189-Rex | Rex-pME-FLAG | Rex(Δaa150-189)-Top: CCCAACCCTGAACTAGTCTAGAGAAA Rex(Δaa150-189)-Bottom: TAGTTCAGGGTTGGGAGGTGCTGCC |
| Δaa150-159-Rex | Rex-pME-FLAG | Rex(Δaa150-159)-Top: CCAACCCAAAACCTGTACACCCTCT Rex(Δaa150-159)-Bottom: CAGGTTTTGGGTTGGGAGGTGCTGCC |
| Δaa160-169-Rex | Rex-pME-FLAG | Rex(Δaa160-169)-Top: CGGCCCCTTGTCTGCATGTACCTCT Rex(Δaa160-169)-Bottom: GCAGACAAGGGGCCGGAGTCCGGGGT |
| Δaa150-169-Rex | Rex-pME-FLAG | Rex(Δaa150-169)-Top: CCAACCCTTGTCTGCATGTACCTCT Rex(Δaa150-169)-Bottom: GCAGACAAGGGTTGGGAGGTGCTGCC |
| Rex-M1 (RRR5/6/7DL) | Rex-pME-FLAG | Rex(M1) -Top: AGACCGATCTGCCCCGCCGATCCCAAAG Rex(M1)-Bottom: GGGGCAGATCGGTCTTGGGCATCTCGAG |
| Rex-M2 (KR14/15DL) | Rex-pME-FLAG | Rex(M2)-Top: AAAGAGATCTACCACCAACACCATGGCC Rex(M2)-Bottom: GTGGTAGATCTCTTTGGGATCGGCGGGG |
| Rex-M3 (FF30/31DL) | Rex-pME-FLAG | Rex(M3)-Top: GAGTCGATCTTTCGGATACCCAGTCTAC Rex(M3)-Bottom: CCGAAAGATCGACTCTGTCCAAACCCTG |
| Rex-M4 (DTQ33/34/35DL) | Rex-pME-FLAG | Rex(M4)-Top: TTTCGGATCTGTCTACGTGTTTGGAGAC Rex(M4)-Bottom: TAGACAGATCCGAAAAGAAGACTCTGTC |
| Rex-M5 (YK43/44DL) | Rex-pME-FLAG | Rex(M5)-Top: CTGTGGATCTGGCGACTGGTGCCCCATC Rex(M5)-Bottom: TCGCCAGATCCACAGTCTCCAAACACGT |
| Rex (T22A) | Rex-pME-FLAG | Rex (T22A)-Top: CCAACACCATGGCCCGCTTCCCAGGGTTTGG Rex (T22A)-Bottom: CCAAACCCTGGGAAGCGGGCCATGGTGTTGG |
| Rex (S36A) | Rex-pME-FLAG | Rex (S36A)-Top: TTTTCGGATACCCAGGCTACGTGTTTGGAGA Rex (S36A)-Bottom: TCTCCAAACACGTAGCCTGGGTATCCGAAAA |
| Rex (T37A) | Rex-pME-FLAG | Rex (T37A)-Top: TCGGATACCCAGTCTGCGTGTTTGGAGACTG Rex (T37A)-Bottom: CAGTCTCCAAACACGCAGACTGGGTATCCGA |
| Rex (S70A) | Rex-pME-FLAG | Rex (S70A)-Top: TGGCCACCTGTCCAGGCCATCAGATCACCTGG Rex (S70A)-Bottom: CCAGGTGATCTGATGGCCTGGACAGGTGGCCA |
| Rex (S97A) | Rex-pME-FLAG | Rex (S97A)-Top: CTCGACTCCCCTCCTGCCCCACCCAGAGAAC Rex (S97A)-Bottom: GTTCTCTGGGTGGGGCAGGAGGGGAGTCGAG |
| Rex (S106A) | Rex-pME-FLAG | Rex (S106A)-Top: GAACCTCTAAGACCCGCAAGGTCCTTACCCC Rex (S106A)-Bottom: GGGGTAAGGACCTTGCGGGTCTTAGAGGTTC |
| Rex (T174A) | Rex-pME-FLAG | Rex (T174A)-Top: CCGTTGTCTGCATGTGCCTCTACCAGCTTTC Rex (T174A)-Bottom: GAAAGCTGGTAGAGGCACATGCAGACAACGG |
| GST- UPF1(G495R/G497E) | GST-UPF1 | hUPF1(G495R/G497E)-Top: AGGGCCCGCCAAGAACGGAGAAGACGGTGAC hUPF1(G495R/G497E)-Bottom: TCACCGTCTTCTCCGTTCTTGGCGGGCCCT |
| GST- UPF1(R843C) | GST-UPF1 | hUPF1(R843C)-Top: TCCTGTCCTGTGTGTGTGCCAACGAGCACCA hUPF1(R843C)-Bottom: TGGTGCTCGTTGGCACACACACAGGACAGGA |
| Rex(-)-pFL-MT2 | pFL-MT2 [44] | p27Rex(ATG-to-CTG)-Top: CCTCAAGCGAGCTGCCTGCCCAAGACCCGTC p27Rex(ATG-to-CTG)-Bottom: GACGGGTCTTGGGCAGGCAGCTCGCTTGAGG |