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. 2022 Feb 15;23(4):2150. doi: 10.3390/ijms23042150

Figure 1.

Figure 1

Cytotoxicity and cell viability assessment (MTT assay). (a) Characterization of gold nanoparticles conjugated with Cur, Pacli, and CP. Upper panel: Transmission electron microscopic (TEM) images of Au-C, Au-P, and Au-CP (scale bar 500 nm). Middle panel: Hydrodynamic dynamic diameter histograms of Au-C, Au-P, and Au-CP obtained by DLS in water. Lower panel: Zeta potential histograms of Au-C, Au-P, and Au-CP obtained by DLS in water. Each formulation was prepared and characterized thrice, and representative histograms are shown. (b) Stability analysis of Au-C (blue line), Au-P (orange line), and Au-CP (grey line) in deionized water at different time points at room temperature. (c) MTT assay (cytotoxicity and cell viability) was performed in cancer cell lines MDA-MB-231, 4T1 by treating with 10.0 and 20.0 μg/mL individually and in the combination of Au-CP at 5.0 and 10.0 μg/mL for 36 h. Similarly, HEK-293 cells were treated with three concentrations (10, 20, and 30 μg/mL). p-value < 0.05, * indicates significance.