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. 2022 Feb 15;23(4):2150. doi: 10.3390/ijms23042150

Figure 2.

Figure 2

(a,b) Apoptotic assay—percentage of cells undergoing apoptosis in different breast cancer cell lines, (a) MDA MB 231 and (b) 4T1, after treatment with Cur, Pacli 10.0 μg/mL, and in the combination of Au-CP at 5.0 μg/mL for 36 h; Annexin V–PI assay). (c,d) DCFDA assay—fluorescence microscopic images of treated (c) MDA-MB 231 and (d) 4T1 cells showed enhancement in the level of green color fluorescent intensity as compared to control untreated cells indicating intracellular ROS accumulation. p-value: <0.05, 0.01, 0.001 indicates * significant, ** very significant, *** highly significant.