Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 24;10(2):260. doi: 10.3390/microorganisms10020260

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The use of SLE tags to monitor bacterial effector secretion or translocation. SLE-tagged effector fusions (i.e., HaloTag, SNAP-tag or CLIP-tag) are expressed in bacteria. Ligand-TMR (bacterial and host cell membrane permeable) addition permits the labeling of bacteria or infected host cells when tracking expressed/secreted or translocated T3E-SLE fusions, respectively. Upon translocation into host cells, the dynamics and subcellular localization of the T3Es under study can be monitored.