Table 2.

Overview of physicochemical properties of the fluorescent reporter systems relying on the FPs GFP, mCherry, or alternatively on LOV:FMN, FlAsH:4Cys or various FAST:fluorogen combinations.

Fluorescent Reporter System	Absorption (nm)	Emission (nm)	Φ (%)	ε (mM−1 cm−1)	KD (µM)	Ref.
eGFP	488	507	60	56	/	/
mCherry	587	610	22	72	/	[77]
LOV:FMN	300–500	450–495	20–40	13	/	[61,78]
4Cys:FlAsH	508	528	49	30–80	10−5	[62,79]
FAST:HMBR	481	540	23	45	0.13	[68]
FAST:HBR-3,5DM	499	562	49	48	0.08	[69]
FAST:HBR-3,5DOM	518	600	31	39	0.97	[69]
FAST:HBRAA-3E	505	559	8	61	1.30	[70]
iFAST:HMBR	480	541	23	41	0.07	[71]
iFAST:HBR-3,5DM	499	558	57	46	0.06	[71]
iFAST:HBR-3,5DOM	516	600	40	38	0.41	[71]
nanoFAST:HBR-DOM2	502	563	56	26	0.85	[72]
FAST:HBR-DOM2	510	566	54	31	0.021	[72]
frFAST:HMBR	484	550	13	56	0.50	[73]
frFAST:HBR-3,5DOM	525	600	19	51	3.90	[73]
frFAST:HPAR-3OM	555	670	21	45	1.00	[73]
greenFAST:HMBR	478	544	23	40	0.09	[74]
redFAST:HBR-3,5DOM	556	603	29	43	1.20	[74]
pFAST:HMBR	481	542	23	54	0.01	[75]
pFAST:HBR-3,5DM	501	561	44	49	0.01	[75]
pFAST:HBR-3,5DOM	520	600	35	44	0.06	[75]
pFAST:HBRAA-3E	506	558	5	53	0.05	[75]