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. 2022 Feb 21;23(4):2391. doi: 10.3390/ijms23042391

Figure 1.

Figure 1

Effect of PTK7 overexpression on the oncogenic properties of ESCC KYSE-30 cells. (A) PTK7 expression in ESCC KYSE-30 cells mock-infected or infected with lentiviruses for vector control (OE Vector) and for expression of PTK7-FLAG and in ESCC TE-10 cells mock-infected was analyzed by Western blotting with PTK7 and GAPDH antibodies. (B) Proliferation of the mock, vector control, and PTK7-overexpressing KYSE-30 cells was analyzed for 3 days. (C) Adhesion of the mock, vector control, and PTK7-overexpressing KYSE-30 cells was analyzed 1 h after plating on dishes coated with type I collagen (1 μg/well). (D) Wound healing of the mock, vector control, and PTK7-overexpressing KYSE-30 cells was analyzed 24 h after the wounding in the monolayer of cells. (E) Chemotactic migration of the mock, vector control, and PTK7-overexpressing KYSE-30 cells was analyzed 24 h after loading of the cells in the upper transwell chamber. The representative images for (C–E) are shown. Each bar represents the mean ± SD from three independent experiments. ** p < 0.01 vs. 0 day (B) or vector control (C–E).