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. 2022 Feb 21;23(4):2391. doi: 10.3390/ijms23042391

Figure 2.

Figure 2

Effect of PTK7 knockdown on the oncogenic properties of ESCC KYSE-30 cells. (A) PTK7 expression in ESCC KYSE-30 cells mock-infected or infected with lentiviruses for vector control (KD Vector) and for PTK7 knockdown shRNA (PTK7-KD-6433 and PTK7-KD-6434) and in ESCC TE-10 cells mock-infected was analyzed by Western blotting with PTK7 and GAPDH antibodies. Proliferation (B), adhesion (C), wound healing (D), and chemotactic migration (E) of the mock, vector control, and PTK7 knockdown shRNA (PTK7-KD-6433 and PTK7-KD-6434) KYSE-30 cells were analyzed using the same methods as in the overexpressing cells. The representative images for (C–E) are shown. Each bar represents the mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. 0 day (B) or vector control (C–E).