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Effect of PTK7 overexpression and knockdown on activation of signaling molecules in ESCC KYSE-30 cells. Tyrosine phosphorylation of cellular proteins, as well as phosphorylation of ERK, AKT, and FAK, was examined by Western blotting in KYSE-30 cells infected with lentiviruses for vector control (OE Vector) and PTK7 overexpression (PTK7-FLAG) (A), as well as the vector control (KD Vector) and PTK7 knockdown shRNA (PTK7-KD-6433 and -6434) (B).
