Figure 1.

Trans-epidermal water loss, mechanical pain, and itch assessments. (A) Bar chart reports quantification of alteration of skin barrier measured in g/m²/h. (B) Bar chart depicts pain sensitivity reported by the participants on a numerical rating scale (NRS) from 0 to 10. (C) Bar chart shows mechanical pain thresholds reported by the subjects. (D) Bar chart shows itching reported on a NRS (0–10) by the subjects. Blue colors represent areas treated with vehicle cream (VC), whereas red colors indicate areas treated with S. ramosissma-based cream (SC). “BS” indicates data obtained at baseline. The label “24 h” reports data relative to group 1 and “48 h” indicates data relative to group 2. “His” and “Cow” indicates data relative to area treated with histamine or cowhage, respectively. Mean and standard deviation of the mean are depicted. *, **, ***, = RM-ANOVA test significance. # = t-test statistical significance.