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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Mol Microbiol. 2021 Oct 18;116(5):1347–1360. doi: 10.1111/mmi.14828

Figure 3. DNA inversion upregulates the expression of CDR20291_0685 in UK_O cells.

Figure 3.

A. The beta-glucuronidase activity of the PCDR20291_0685 upstream-gusA fusions locked in UK1_T and UK1_O orientation in the UK1 parent strain. The data represent the results from three biological replicates. Data were analyzed using 2-way ANOVA (Sidak’s multiple comparisons test) comparing the mean of UK1_T and UK1_O at each time point. *** indicates p<0.005. B. qRT-PCR results showing overexpression of CDR20291_0685 in UK1_O strain at 16h time point. The representative results from three independent experiments are shown. Data were analyzed by a two-tailed unpaired t-test with Welch’s correction where *** is p<0.005.