Skip to main content
. Author manuscript; available in PMC: 2022 Dec 22.
Published in final edited form as: J Am Chem Soc. 2021 Dec 13;143(50):21416–21424. doi: 10.1021/jacs.1c11109

Figure 4.

Figure 4.

Reactivity of BesC-P with Lys derivatives. (A) Substrate consumption in single-turnover reactions of BesC with 4-Cl-Lys, l-Lys, and their perdeuterated analogs. (B and inset) UV–visible spectra show that BesC-P decay with l-Lys occurs slowly and can be fit to a single exponential decay phase with an apparent 2H KIE. (C) BesC-P decay with 4-Cl-Lys and its perdeuterated analog as monitored at 612 nm. The raw data is shown in black, and the fits to a double-exponential expression are shown in blue (protiated) and red (deuterated). The residuals are shown in the corresponding color. Protein concentration used in (B) and (C) was 300 μM with 5 mol equiv substrate.