Figure 9. Reduced B16 melanoma growth in adult induced EPHB4-deficient mice associated with impaired tumor angiogenesis.

Adult TM-treated Ephb4^fl/fl and Ephb4^fl/fl Ub^ert2cre mice were injected in flanks with B16 melanoma cells and tumors were harvested 13 days later. Some mice received 4PBA at the same time as the tumor and on all subsequent days until tumor harvest. (A) Representative images of explanted tumors. (B) Plot shows tumor weights at day 13. Bars represent mean ± 1 SEM tumor weight (Ephb4^fl/fl TM alone, n = 14; Ephb4^fl/fl Ub^ert2cre TM alone, n = 18; Ephb4^fl/fl TM + 4PBA, n = 12; Ephb4^fl/fl Ub^ert2cre TM + 4PBA, n = 18). (C) Sections of explanted tumors were stained with anti-CD31 antibodies to identify BVs. Shown are representative images. (D) Plot shows percentage BV coverage of randomly selected 200 × 200 μm areas of tumors. Bars represent mean ± 1 SEM percentage BV coverage (Ephb4^fl/fl TM alone, n = 26; Ephb4^fl/fl Ub^ert2cre TM alone, n = 27; Ephb4^fl/fl TM + 4PBA, n = 18; Ephb4^fl/fl Ub^ert2cre TM + 4PBA, n = 35). *, P < 0.05; **, P < 0.01; ****, P < 0.0001; 1-way ANOVA with Tukey.