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. 2022 Feb 22;7(4):e150723. doi: 10.1172/jci.insight.150723

Figure 5. Chronic AA effects on kidney macrophage phenotype.

Figure 5

(A) Renal CD68 protein abundance in control and macrophage IRF4–/– animals following AA administration. (B) Total kidney CD68 and F4/80 mRNA abundance following AA administration. Five fields of each kidney section were randomly selected, and the total number of DAB-positive cells per field was counted in a blinded fashion. Effects of chronic AA administration on (C) proinflammatory (TNF-α, IL-23α, IL-1α, IL-1β, CCL2, and CCL3) and (D) proresolving (IL-4Rα, Fizz1, IL-10, and CD206) mRNA abundance in isolated macrophages from control and macrophage IRF4–/– animals. For A, n = 5 per group. For B–D, n = 8 per group. **P < 0.05 by unpaired Student’s t test. AA, aristolochic acid; Mϕ, macrophage. Scale bar: 50 μM. Note that all animals were treated with AA.