FIG. 2.

IL-1β and dexamethasone (Dex) acetylate specific and distinct lysine residues. Immunocytochemical staining for specific histone H4 acetylated lysine residues. Cells were incubated with dexamethasone (10⁻⁷ M) (b, f, j, and n), IL-1β (1 ng/ml) (c, g, k, and o), or TSA (100 ng/ml) (d, h, l, and p) for 6 h before staining for acetylated forms of histone H4 lysine residues K5 (a to d), K8 (e to h), K12 (i to l), and K16 (m to p). Results are representative of four independent experiments.