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. 2022 Feb 22;7(4):e140738. doi: 10.1172/jci.insight.140738

Figure 4. Commensal oral microbiota has pro-osteoclastic actions distinct from the systemic microbiota, which enhance osteoclast numbers lining alveolar BM.

Figure 4

(A–H) Sagittal sections of the maxillary first molar were TRAP stained for osteoclast cellular outcomes in (A–D) SPF versus GF mice (n = 6/gp) and (E–H) saline versus CHX mice (n = 6/gp). (A) Representative TRAP stain in the maxillary first molar furcation of SPF versus GF mice (×200). (E) Representative TRAP stain in the maxillary first molar furcation of saline versus CHX mice (×200). (I–P) Frontal sections of proximal tibia TRAP stained for osteoclast cellular outcomes in (I–L) SPF versus GF mice (n = 6/gp) and (M–P) saline versus CHX mice (n = 6/gp). (I) Representative TRAP stain in proximal tibia of SPF versus GF mice (×200). (M) Representative TRAP stain in proximal tibia of saline versus CHX mice (×200). (Q–S) nCounter analysis of osteoclast genes and signaling factors in the alveolar BM (ABM) and long BM (LBM) of (Q–S) SPF versus GF mice (n = 4/gp). (T–V) nCounter analysis of osteoclast genes and signaling factors in the ABM and LBM of saline versus CHX mice (n = 6/gp). Unpaired t test; data reported as mean ± SEM; *P < 0.05, **P < 0.01.