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. 2022 Feb 22;7(4):e150894. doi: 10.1172/jci.insight.150894

Figure 3. Zfp277 deficiency attenuates epithelial cell proliferation in murine small intestine and colon.

Figure 3

(A) Zfp277 deficiency reduces small intestinal Ki67 expression. Brackets indicate staining at the base of the crypts. Representative Ki67 IHC staining of ileal segments from mice with the indicated genotypes. (B) Numbers of Ki67+ cells per ileal crypt in mice of the indicated genotype. (C) Representative Ki67 IHC staining of distal colons from mice with the indicated genotype. Values represent mean ± SEM from 3 mice for each genotype. (D) Numbers of Ki67+ cells per colon crypt. All mice were 15-week-old males. Scale bar: 100 μM. Values represent mean ± SEM from 3 mice in each genotype. Data were analyzed using 1-way ANOVA. (E) Representative confocal microscopy images of BrdU (green; 2-hour labeling) and Ki67 (red) IF staining and merged images of BrdU, Ki67, and DAPI of ileal segments from 15-week-old male mice with the indicated genotype. Arrows indicate fluorescence signals at the base of the crypts. Scale bar: 100 μM.