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. 2022 Feb 22;7(4):e150894. doi: 10.1172/jci.insight.150894

Figure 6. ZNF277 expression is regulated by β-catenin and ZNF277 association with BMI1 in human colon cancer cells.

Figure 6

(A) β-Catenin (CTNNB1) knockdown decreases ZNF277 levels in human HT29, H508, and SNUC4 colon cancer cells. (B) ZNF277 knockdown does not alter β-catenin levels in HT29 and H508 colon cancer cells. Maximum siRNA concentration was 50 μM. (C) Increased β-catenin signaling augments Zfp277 expression. Zfp277 expression in colon mucosal extracts from WT, ApcMin/+, and Zfp277–/– mice. β-Actin was used as a loading control. (D) ZNF277 promoter elements for β-catenin. ChIP assay using random DNA fragments generated by MNase digestion in HT29 cells. ZNF277 promoter positions are indicated in the text and DNA sequences of qPCR primers are listed in Supplemental Table 2. Data are shown as mean ± SEM from 3 separate experiments. (E) ZNF277 coimmunoprecipitates with BMI1 in SNUC4 colon cancer cells. Rabbit immunoglobulins (IgG) were used as control.