Figure 1.

AIM2 expression is controlled by the TCR. (a) Total RNA samples from two different subjects (S1 and S2) were subjected to qRT-PCR for the amplification of Aim2 mRNA and visualized on an agarose gel after 30 cycles. NTCs were run on the same gel but were not contiguous. (b) Flow cytometry analysis of AIM2 expression in CD3⁺CD4⁺ T cells from peripheral blood mononuclear cells (PBMCs) of three different subjects (S1–S3). (c) Western blot analysis of AIM2 expression in CD4⁺ cells purified from PBMCs of two different subjects (S1–S2). (d) Total RNA samples from four different mouse CD4⁺ T cell samples (S1–S4) were subjected to qRT-PCR for the amplification of Aim2 mRNA and visualized on an agarose gel after 30 cycles. (e) Western blot analysis of AIM2 expression in mouse spleen CD4⁺ T cells. CD4⁺ T cells were stimulated with anti-CD3 and anti-CD28 (αCD3/28) antibodies with or without LPS pre-stimulation (2 h) and total protein extracts were collected at the indicated timepoints. Anti-mouse AIM2 and anti-mouse β-actin primary antibodies were used after immunoblot for detection of AIM2 expression and loading control, respectively. (f) Quantification of blot images performed using ImageJ software (au; arbitrary units). Data were analyzed using Sidak’s multiple comparison test and are displayed as scatter plot with median ± range; * p < 0.05. Data are representative of three different experiments with similar results. NTC = non-template control.