Mean muscle activity value comparison in microvolts (µV) of the masseter muscle in different jaw positions, where RM is relaxed mandible, MI is maximum intercuspation position, CR is jaw in centric relation according to Dawson’s bimanual manipulation, CR-L is centric relation measured using Long’s laminae, CR-P is centric relation obtained in the position of the first dental contact, DT is jaw disclusion time, P is jaw protrusion position and T-MI is time needed to reach the position of maximum intercuspation. We were able to clearly observe a reduction in activity in the right (19 µV) and left (20.36 µV) masseter muscles in a relaxed jaw position before needling the trigger points. After waiting 10 min post-needling and repeating the same measurement, we obtained values of 23.71 µV/17.65 µV, respectively.