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. 2022 Jan 24;14(2):221. doi: 10.3390/v14020221

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Recombinant salivary proteins AAEL000793, AAEL006347 and AAEL003601 induce antibody response in BALB/c mice. Six-week-old female BALB/c mice (5 per group) were inoculated with 50 µg of recombinant AAEL000793, AAEL006347 or AAEL003601. An enzyme-linked immunosorbent assay (ELISA) was conducted following the final bleed to measure the resulting IgG antibody levels, and any cross-reactivity between proteins and their specific antibodies. Antibodies levels were determined by ELISA assays. Mice immunized with Magic Mouse Adjuvant alone were used as a negative control. Bars show means of optical density values of all the proteins. OD = optical density.