FIG. 3.

(A) to mRNA is colocalized with tim and exhibits cycling expression in adult head sections. Shown are in situ hybridizations of antisense digoxigenin RNA probes for to, tim, Clk, and cyc, frontal sections of adult head at ZT13 and ZT23. Arrows point to where the lateral neurons are located. The mRNA expression of to and tim cycles with a different phase. Expression of both to and tim was detected in the photoreceptors and brain cortex, especially in the optic lobe regions. tim has broader expression, e.g., in the glial cells and in the central body of the central complex. Clk and cyc have the same expression pattern as tim. In situ hybridizations using sense RNA probes did not show obvious signals. (B) Alignment of the to family members. Boxes indicate the signature motifs that define the to family. Out of the 10 family members identified so far, 7 that have full-length or almost full-length conceptual translation sequences are shown. Five of the members, including to and 0.9kb (GenBank accession number AL024485), AA696925, and AA142273, are from Drosophila melanogaster. Two, including AI142207, are from Manduca sexta. Three, including AU002769 and AU004740, are from Bombyx mori. The original EST sequences were obtained from the BDGP/HHMI Drosophila EST project. The EST sequences shown here are refinements from the different clone sequences, and accession numbers of the representative ones are used. Black background with white letters indicates identity; grey background with black letters indicates similarity.