Figure 2.

Intra- and interrater variability of TIL scores determined on antibody- and HE-stained tissue sections. (A) Staining of breast cancer tissue sections with antibodies specific for CD45, CD3, CD4, or CD8. Scale bars: 100 µm. (B) Graph showing intrarater variability. (C) Graph showing interrater variability. (D) Box plots showing method variability. Data information: 37 biopsies were stained with HE or immune cell-specific antibodies and scored twice with a washout period of 3 days by three independent pathologists. In (B, C), data are presented as mean ± confidence interval of 95%.