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. 2022 Feb 11;13:794175. doi: 10.3389/fimmu.2022.794175

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Copyright © 2022 Locy, Verhulst, Cools, Waelput, Brock, Cras, Schiettecatte, Jonckheere, van Grunsven, Vanhoeij, Thielemans and Breckpot

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TIL score calculated on RUVSeq-normalized expression data correlates with TIL-score based on immunohistochemistry. (A) Box plots showing raw counts obtained from nCounter MAX analysis, normalized counts using nSolver software, and normalized counts using RUVSeq package. (B) Graph showing the correlation between nSolver- or RUVSeq-calculated TIL scores. (C) Heatmap showing Pearson correlation between different TIL scoring methods. Data information: 12 breast cancer CNB samples were used to extract RNA for downstream gene expression profiling.