Figure 2.

Construction of recombinant Marek’s disease viruses. (A) Schematic diagrams of the constructs cloned the RB-1B genome used in this study. In the RB-1B genome cloned as the bacterial artificial chromosome (BAC) plasmid (pRB-1B), most of the internal repeat long (IRL) regions were deleted, designated as pRB-1B_ΔIRL, and used for mutagenesis. The meq gene in terminal repeat long (TRL) was replaced with the RB-1B-meq, short-Meq containing the deletion (S-meq) (RB-1B), or long-Meq containing the insertion (L-meq) (RB-1B) genes by two-step red-mediated mutagenesis. (B) Restriction fragment length polymorphism analysis of the BAC plasmids obtained by mutagenesis. The BAC plasmids were digested with BamHI to determine the accurate insertion of each meq-isoform into the RB-1B genome. A dashed box indicates the variation of the recombinant Marek’s disease virus genomes with each meq-isoform.