Table 1.

Introduction of the mutation into the S-meq and/or L-meq genes.

Position in Meq	Substitution	Primers
71	serine–to–alanine	F	5′-GAA TCG TGA CGC CGC TCG GAG AAG ACG-3′
		R	5′-CGT CTT CTC CGA GCG GCG TCA CGA TTC-3′
77, 80	glutamic-acid–to–lysine, tyrosine–to–aspartic-acid	F	5′-CGG AGA AGA CGC AGG AAG CAG ACG GAC TAT GTA GAC AAA C-3′
		R	5′-GTT TGT CTA CAT AGT CCG TCT GCT TCC TGC GTC TTC TCC G-3′
114, 115	cysteine–to–arginine, alanine–to–valine	F	5′-GAG TGC ACG TCC CTG CGT GTA CAG TTG GCT TGT CA-3′
		R	5′-TGA CAA GCC AAC TGT ACA CGC AGG GAC GTG CAC TC-3′
217	alanine–to–proline	F	5′-ATC TGT ACC CCC CCT CCT CCC GAT G-3′
		R	5′-CAT CGG GAG GAG GGG GGG TAC AGA T-3′
326	isoleucine–to–threonine	F	5′-GTT TCC CTC GGA TAC TCA GTC TAC GGT CT-3′
		R	5′-AGA CCG TAG ACT GAG TAT CCG AGG GAA AC-3′