FIG. 4.

Inhibition of PI3K with wortmannin results in a shift of plasma membrane-associated Itk into the cytoplasm. JTAg cells expressing GFP-tagged Itk were treated with vehicle (0.1% dimethyl sulfoxide) or 100 nM wortmannin and examined by fluorescence confocal microscopy at 1, 2, 3, and 4 h after the beginning of treatment. To better visualize the cytoplasmic compartment, the nuclei were stained with Hoechst 33342.