Table 1.
List of bacterial isolates retrieved from farmed Platax orbicularis affected by ‘white-patch’ disease, with their sources and characteristics. Virulence was evaluated by experimental bath challenge on T. maritimum-free batfish. Significant differences (p < 0.05) in mortality rate (see paragraph 3-3) between non-infected and infected fish are indicated by ‘yes’ or ‘no’. Isolate identification was performed using the EzBioCloud software [30], based on >99% identity of their 16S rRNA sequences with the closest type strain. ST refers to the MLST sequence type. n/a: not analysed.
| Isolate | Source and Date of Isolation | GPS Location | 16S rDNA GenBank Accession Number | Bacterial Species | Virulence | ST |
|---|---|---|---|---|---|---|
| TFA4 | Skin lesions, Tautira lagoon, Tahiti, 2013 | 17°47′50″ S, 149°07′14″ W | MW690171 | T. maritimum | yes | ST168 |
| Aq 9–66 | Skin lesions, Tautira lagoon, Tahiti, 2013 | 17°47′50″ S, 149°07′14″ W | MW690177 | T. mesophilum | no | |
| Aq 9–67 | Skin lesions, Tautira lagoon, Tahiti, 2013 | 17°47′50″ S, 149°07′14″ W | MW690178 | T. mesophilum | no | |
| Aq 16–83 | Skin lesions, Vairao lagoon, Tahiti, 2016 | 17°48′22″ S, 149°17′36″ W | MW690172 | T. maritimum | n/a | n/a |
| Aq 16–84 | Skin lesions, Vairao lagoon, Tahiti, 2016 | 17°48′22″ S, 149°17′36″ W | MW690173 | T. maritimum | yes | n/a |
| Aq 16–85 | Skin lesions, Vairao lagoon, Tahiti, 2016 | 17°48′22″ S, 149°17′36″ W | MW690174 | T. maritimum | n/a | ST167 |
| Aq 16–87 | Skin lesions, Vairao lagoon, Tahiti, 2016 | 17°48′22″ S, 149°17′36″ W | MW690175 | T. maritimum | yes | n/a |
| Aq 16–88 | Skin lesions, Vairao lagoon, Tahiti, 2016 | 17°48′22″ S, 149°17′36″ W | MW690176 | T. maritimum | n/a | ST167 |
| Aq 16–89 | Skin lesions, Vairao lagoon, Tahiti, 2016 | 17°48′22″ S, 149°17′36″ W | MW690180 | T. maritimum | n/a | ST167 |
| Aq 16–91 | Skin lesions, Vairao lagoon, Tahiti, 2016 | 17°48′22″ S, 149°17′36″ W | MW690179 | T. mesophilum | n/a |