Figure 2.

Glucosamine supplementation confers tolerance to several ER stress inducers and to mutants in different signaling pathways. (A) Drop test of BY4741 wild-type (wt), hog1, and slt2 mutant strains. Cultures were diluted (1–10⁻³) and spotted (2 μL) onto YPD plates lacking or containing 11.5 mM glucosamine (GlcN) and/or 0.5 μg/mL tunicamycin (Tn). (B) Cultures of the indicated strains, BY4741 wild type (wt), slt2, ire1, and hac1, were assayed for growth on YPD plates lacking or containing GlcN at the indicated concentrations and/or 0.5 μg/mL Tn or 17 mM dithiothreitol (DTT). (C) The ER-stress tolerance of the BY4741 wild-type (wt), reg1, snf1, and reg1 snf1 strains was inspected on YPD plates lacking or containing glucosamine (GlcN) and/or tunicamycin (Tn). Drug concentrations and cultures processing conditions were as in panel (A). In all cases, a representative experiment is shown.