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. 2022 Jan 18;8(2):92. doi: 10.3390/jof8020092

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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TORC1 activity is not inhibited by tunicamycin in absence of Slt2. Analysis of TORC1 activity as measured by phosphorylation of the 40S ribosomal protein S6. Protein extracts obtained from YPD-grown cultures (OD₆₀₀ ~ 0.5) were treated with 2 μg/mL tunicamycin (Tn) for the indicated times in the presence (+) or absence (−) of 11.5 mM glucosamine (GlcN), separated by SDS-PAGE and analyzed by Western blot for phospho-Rps6 (P-Rps6). The level of glucose-6-phosphate dehydrogenase (G6Pdh) was used as a loading control for crude extracts. A representative experiment is shown.